Haynes Lab:Notebook/CRISPR Editing/2014/09/22: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/09/22 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
Rene M Davis (talk | contribs) |
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== | ==09/22/2014== | ||
Cut the annealed Luc14 reactions, but not gRNA 21 because that one didn't amplify. added 40ul of annealed reaction to a new PCR tube, added 1ul of enhancer and 2ul of Surveyor nuclease. Incubated at 42°C for one hour, kept on ice after.<br> | |||
Ran gel: | |||
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Added 100ul of elution butffer | |||
Revision as of 13:20, 22 September 2014
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09/22/2014Cut the annealed Luc14 reactions, but not gRNA 21 because that one didn't amplify. added 40ul of annealed reaction to a new PCR tube, added 1ul of enhancer and 2ul of Surveyor nuclease. Incubated at 42°C for one hour, kept on ice after.
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