Haynes Lab:Notebook/CRISPR Editing/2014/09/21: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/09/21 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==09/21/2014== | ||
Ran the PCR for gRNA 20 samples from yesterday on a gel. Ran 3μL of each sample and 2μL and 1μL of ladder. Only the first three worked, even though the other two worked the other day. | |||
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Annealing all of each of gRNA 20, plus the PCRs from the other day that worked. Will just go through the protocol to see if I can even do Surveyor with the concentrations I'm getting. | |||
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Going to try more PCR. Will do gRNA 21 samples and all the Luc14 samples. If I can get all the Luc14 samples through the entire protocol, I can know if any of the gRNAs aren't cutting at all and won't have to keep trying the chromatin-induced samples. | |||
Revision as of 13:30, 21 September 2014
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09/21/2014Ran the PCR for gRNA 20 samples from yesterday on a gel. Ran 3μL of each sample and 2μL and 1μL of ladder. Only the first three worked, even though the other two worked the other day.
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