Haynes Lab:Notebook/CRISPR Editing/2014/09/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 11: Line 11:


<br><br>
<br><br>
PCR gradient of a sample that didn't have a band on the last gel. Gradient from 66-56°C. Didn't see any bands at all.  
PCR gradient with no DMSO of a sample that didn't have a band on the last gel. Gradient from 66-56°C. Didn't see any bands at all.  
<br><br>
<br><br>


Latest revision as of 00:19, 27 September 2017

Project name Main project page
Previous entry      Next entry

09/18/2014

Poured large gel with tiny combs. Ran dilutions of ladder and PCR products from last night. Some of them worked, some didn't. Possible the DMSO is messing things up.



PCR gradient with no DMSO of a sample that didn't have a band on the last gel. Gradient from 66-56°C. Didn't see any bands at all.

Samples from gRNA 20 all had bands. Can at least test those. Should redo the PCR though. Will do one more test PCR tonight and then do gRNA 20 samples tomorrow.

Test: run a gradient PCR of a sample that I've had success with before with and without DMSO.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2014/09/18&oldid=1015372"