Haynes Lab:Notebook/CRISPR Editing/2014/09/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 21: Line 21:
|-
|-
| SURVEYOR Ctrl G PCR reaction||0.053||0.028||1.877||53.041
| SURVEYOR Ctrl G PCR reaction||0.053||0.028||1.877||53.041
|-
|
|}
|}
<br>
<br>

Revision as of 09:29, 15 September 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

09/15/2014

Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction. Ran binding through twice and water elution through twice. Eluted in 30ul.

Sample Read# 260 280 260/280 ng/µL
gRNA 20 +dox rep 1' PCR reaction with P197/P198 0.003 0.001 3.444 3.212
SURVEYOR Ctrl C PCR reaction 0.066 0.035 1.9 65.558
SURVEYOR Ctrl G PCR reaction 0.053 0.028 1.877 53.041


Ran 6 ul on gel.


Gel
Top

Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction.

Bottom

Luc->Cyan PCR reactions
pure 1, 2a, 2b, pure 3a, pure 3b, 4a, 4b, pure 4c, 4c3


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2014/09/15&oldid=820408"