Haynes Lab:Notebook/CRISPR Editing/2014/09/15

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09/15/2014

Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction. Ran binding through twice and water elution through twice. Eluted in 30ul.

Sample Read# 260 280 260/280 ng/µL
gRNA 20 +dox rep 1' PCR reaction with P197/P198 0.003 0.001 3.444 3.212
SURVEYOR Ctrl C PCR reaction 0.066 0.035 1.9 65.558
SURVEYOR Ctrl G PCR reaction 0.053 0.028 1.877 53.041


Ran 6 ul on gel.


Gel
Top

Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction.

Bottom

Luc->Cyan PCR reactions
pure 1, 2a, 2b, pure 3a, pure 3b, 4a, 4b, pure 4c, 4c3


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