Haynes Lab:Notebook/CRISPR Editing/2014/09/15: Difference between revisions

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==09/15/2014==
==09/15/2014==
Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction. Ran binding through twice and water elution through twice. Eluted in 30ul. Ran 6 ul on gel.
Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction. Ran binding through twice and water elution through twice. Eluted in 30ul.  
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
|-
| gRNA 20 +dox rep 1' PCR reaction with P197/P198||0.003||0.001||3.444||3.212
|-
| SURVEYOR Ctrl C PCR reaction||0.066||0.035||1.9||65.558
|-
| SURVEYOR Ctrl G PCR reaction||0.053||0.028||1.877||53.041
|-
|
|}
<br>
Ran 6 ul on gel.


<br>
<br>

Revision as of 09:29, 15 September 2014

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09/15/2014

Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction. Ran binding through twice and water elution through twice. Eluted in 30ul.

Sample Read# 260 280 260/280 ng/µL
gRNA 20 +dox rep 1' PCR reaction with P197/P198 0.003 0.001 3.444 3.212
SURVEYOR Ctrl C PCR reaction 0.066 0.035 1.9 65.558
SURVEYOR Ctrl G PCR reaction 0.053 0.028 1.877 53.041


Ran 6 ul on gel.


Gel
Top

Purified 'gRNA 20 +dox rep 1' PCR reaction with P197/P198, SURVEYOR Ctrl C PCR reaction, and SURVEYOR Ctrl G PCR reaction.

Bottom

Luc->Cyan PCR reactions
pure 1, 2a, 2b, pure 3a, pure 3b, 4a, 4b, pure 4c, 4c3


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