Haynes Lab:Notebook/CRISPR Editing/2014/09/10: Difference between revisions
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==09/10/2014== | ==09/10/2014== | ||
Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow. | Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow.<br><br> | ||
Expected band sizes: | |||
:1522 for lanes 1 & 3 | |||
:1660 for lanes 2 & 4 | |||
<br> | |||
[[Image:14.09.10_C_on_Luc_SRVYR_primer_test.png|420px]] | |||
<br><br> | <br><br> | ||
Checked Luc->AmCyan PCR donor I transfected with on a gel. The correct band is there but | Checked Luc->AmCyan PCR donor I transfected with on a gel. The correct band is there but is faint with other bands so I will do the PCR again and raise the at and reduced the amount of template to try to get rid of other bands.<br> | ||
[[Image:14.09.10_Luc_to_AmCyan_donor.png|300p]] | |||
Ran PCR of PCR 4c 2 (PCR 4c 3), 100μL reaction, 1μL of each primer, .5μL template. | Ran PCR of PCR 4c 2 (PCR 4c 3), 100μL reaction, 1μL of each primer, .5μL template. | ||
Revision as of 09:06, 11 September 2014
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09/10/2014Did a test PCR for CRISPR on chromatin samples. Looks great, will start with all samples tomorrow.
Ran PCR of PCR 4c 2 (PCR 4c 3), 100μL reaction, 1μL of each primer, .5μL template.
Annealing Temp 57, et 1min
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