Haynes Lab:Notebook/CRISPR Editing/2014/09/02: Difference between revisions
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<br><br> | |||
Ran 3a and 3b PCR products on gel, both bands were there. 3a was really bright but both lanes had lots of background. <br> | |||
Purified the PCR products using Sigma kit.<br> | |||
PCR | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''Template''' | |||
| align="center" style="background:#f0f0f0;"|'''F primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Exp band''' | |||
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| 4a||3a||P179||P182||962 | |||
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| 4b||3b||P179||P182||962 | |||
|- | |||
| 4c||3b||P192||P195||918 | |||
|} | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 11:47, 2 September 2014
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09/02/2014Miniprepped colonies picked yesterday from ligation plates that were left out for 72 hours.
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