Haynes Lab:Notebook/CRISPR Editing/2014/09/02: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/09/02 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==09/02/2014== | ||
Miniprepped colonies picked yesterday from ligation plates that were left out for 72 hours. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample Read#''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| gRNA 26||0.028||0.013||2.102||27.876 | |||
|- | |||
| gRNA 30||0.022||0.008||2.556||21.644 | |||
|} | |||
<br><br> | |||
Ran 3a and 3b PCR products on gel, both bands were there. 3a was really bright but both lanes had lots of background. <br> | |||
Purified the PCR products using Sigma kit.<br> | |||
PCR | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''Template''' | |||
| align="center" style="background:#f0f0f0;"|'''F primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Exp band''' | |||
|- | |||
| 4a||3a||P179||P182||962 | |||
|- | |||
| 4b||3b||P179||P182||962 | |||
|- | |||
| 4c||3b||P192||P195||918 | |||
|} | |||
Annealing Temp 55, et 1min | |||
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|} | |} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 00:13, 27 September 2017
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09/02/2014Miniprepped colonies picked yesterday from ligation plates that were left out for 72 hours.
Annealing Temp 55, et 1min |