Haynes Lab:Notebook/CRISPR Editing/2014/09/01: Difference between revisions

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==07//2014==
==09/01/2014==
 
Cameron ran gel of PCR products for making Luc->AmCyan donor DNA. Bands were between 700-800bp.
 
<br>
Ran PCR on 2b, AT 49°C and et 1min
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
| align="center" style="background:#f0f0f0;"|'''Exp band'''
|-
| 3a||2b||191||194||834
|-
| 3b||2b||178||181||877
|}


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Revision as of 11:40, 2 September 2014

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09/01/2014

Cameron ran gel of PCR products for making Luc->AmCyan donor DNA. Bands were between 700-800bp.
Ran PCR on 2b, AT 49°C and et 1min

Sample Template F primer R primer Exp band
3a 2b 191 194 834
3b 2b 178 181 877