Haynes Lab:Notebook/CRISPR Editing/2014/08/28: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==08/29/2014== | ||
Annealed gRNAs 26 and 30. Cut and ligated into pX330. Transformed into DH5αTs. | |||
<br><br> | |||
PCR cleaned the Luc-AmCyan PCR 1 from yesterday using Sigma PCR cleanup kit.<br> | |||
Second round of PCR | |||
*2a - P185, P188 | |||
*2b - P190, P188 | |||
<br> | |||
Ran on Wang lab machine, AT 44°C, ET 60s | |||
<br> | |||
Spun down liquid cultures started yesterday, put at 4deg. | |||
Latest revision as of 00:12, 27 September 2017
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08/29/2014Annealed gRNAs 26 and 30. Cut and ligated into pX330. Transformed into DH5αTs.
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