Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
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Assistance from Week 2 Team 2 | Assistance from Week 2 Team 2 | ||
PCR primers | '''PCR primers''' | ||
{| | {| {{table}} | ||
|- | |- | ||
| Target || Genomic DNA (template) || Primers || Amplicon | | Target || Genomic DNA (template) || Primers || Amplicon | ||
|- | |||
| mCherry gRNA1d || CRISPR 1d || q1F / P3R || 329 bp | | mCherry gRNA1d || CRISPR 1d || q1F / P3R || 329 bp | ||
|} | |} | ||
* Annotated map at https://benchling.com/cshlsynbio/mammalian-crispr/Rc6-kah154/edit | * Annotated map at https://benchling.com/cshlsynbio/mammalian-crispr/Rc6-kah154/edit | ||
Revision as of 09:10, 7 August 2014
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08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
SURVEYOR assay PCR primers
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