Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
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| -dox || 1.169 || 1.8 || 58.5 | | -dox || 1.169 || 1.8 || 58.5 | ||
|- | |- | ||
| | | U2OS mock || --- || --- || not done yet | ||
|} | |} | ||
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'''Mismatch annealing''' | '''Mismatch annealing''' | ||
* Zhang Lab approach - do not mix with a wild type reference, since ~50% or more of the amplicons in the mutated cell sample will be wild type anyway | * Zhang Lab approach - do not mix with a wild type reference, since ~50% or more of the amplicons in the mutated cell sample will be wild type anyway | ||
* 200 ng clean PCR, 1x HF Phusion buffer, in final volume of 20 μL | ** 200 ng clean PCR, 1x HF Phusion buffer, in final volume of 20 μL | ||
# CRISPR 1d | # CRISPR 1d | ||
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# g20 +dox | # g20 +dox | ||
# g20 -dox | # g20 -dox | ||
# g29 +dox | # g29 +dox | ||
# g29 -dox | |||
# +dox | |||
# -dox | |||
# U2OS mock | |||
Revision as of 07:30, 8 August 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
SURVEYOR assay
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