Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions

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(Autocreate 2014/08/07 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==07//2014==
==08/07/2014==


* CRISPR on chromatin: transfections of g21, g22, g27 into Gal4EED/luc cells
* Luc-mCh swap
----
'''CRISPR on chromatin: transfections'''<br>
# gRNA21/Cas9, dox+
# gRNA22/Cas9, dox+
# gRNA27/Cas9, dox+
# gRNA21/Cas9, dox-
# gRNA22/Cas9, dox-
# gRNA27/Cas9, dox-
* On 8/05/14, cells were grown in 4 mL P/S-free medium to ~80% confluence in a 6-well plate, 3 with 1 μg/mL dox, 3 without dox




Revision as of 08:30, 7 August 2014

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08/07/2014

  • CRISPR on chromatin: transfections of g21, g22, g27 into Gal4EED/luc cells
  • Luc-mCh swap


CRISPR on chromatin: transfections

  1. gRNA21/Cas9, dox+
  2. gRNA22/Cas9, dox+
  3. gRNA27/Cas9, dox+
  4. gRNA21/Cas9, dox-
  5. gRNA22/Cas9, dox-
  6. gRNA27/Cas9, dox-
  • On 8/05/14, cells were grown in 4 mL P/S-free medium to ~80% confluence in a 6-well plate, 3 with 1 μg/mL dox, 3 without dox



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