Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
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'''Mismatch annealing''' (continued on 08/07/14) | |||
'''Mismatch annealing''' | |||
* Zhang Lab approach - do not mix with a wild type reference, since ~50% or more of the amplicons in the mutated cell sample will be wild type anyway | * Zhang Lab approach - do not mix with a wild type reference, since ~50% or more of the amplicons in the mutated cell sample will be wild type anyway | ||
** 200 ng clean PCR, 1x HF Phusion buffer, in final volume of 20 μL | ** 200 ng clean PCR, 1x HF Phusion buffer, in final volume of 20 μL | ||
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** 4 ºC/ hold ∞ | ** 4 ºC/ hold ∞ | ||
'''SURVEYOR nuclease reaction''' | |||
Latest revision as of 00:10, 27 September 2017
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08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
SURVEYOR assay
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