Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
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| mCherry gRNA2 || CRISPR 2d || " || 329 || ~52, 277 | | mCherry gRNA2 || CRISPR 2d || " || 329 || ~52, 277 | ||
|- | |- | ||
| mCherry gRNA4 || CRISPR | | mCherry gRNA4 || CRISPR 4d || " || 329 || ~180, 149 | ||
|- | |- | ||
| mCherry || blank || " || 329 || no cutting (PCR already done) | | mCherry || blank || " || 329 || no cutting (PCR already done) | ||
|- | |- | ||
| luciferase gRNA20 || g20 || gRNA 021 US P F / gRNA 027 ES P R || 1295 || ~165, 1130 | | luciferase gRNA20 || g20 +dox || gRNA 021 US P F / gRNA 027 ES P R || 1295 || ~165, 1130 | ||
|- | |- | ||
| luciferase | | luciferase gRNA20 || g20 -dox || " || 1295 || ~165, 1130 | ||
|- | |- | ||
| luciferase || mock || " || 1295 || no cutting | | luciferase gRNA29 || g29 +dox || " || 1295 || ~395, 900 | ||
|- | |||
| luciferase gRNA29 || g29 -dox || " || 1295 || ~395, 900 | |||
|- | |||
| luciferase || mock +dox || " || 1295 || no cutting | |||
|- | |||
| luciferase || mock -dox || " || 1295 || no cutting | |||
|} | |} | ||
Revision as of 11:01, 7 August 2014
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08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
SURVEYOR assay
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