Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
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Line 90: | Line 90: | ||
| Sample || OD 260 || 260/280 || ng/uL | | Sample || OD 260 || 260/280 || ng/uL | ||
|- | |- | ||
| CRISPR 1d || | | CRISPR 1d || 1.562 || 1.81 || 78.1 | ||
|- | |- | ||
| CRISPR 2d || | | CRISPR 2d || 0.273 || 1.71 || 14.0 | ||
|- | |- | ||
| CRISPR 4d || | | CRISPR 4d || 1.77 || 1.588 || 79.3 | ||
|- | |- | ||
| | | g20 +dox || 1.408 || 1.79 || 70.4 | ||
|- | |- | ||
| | | g20 -dox || 1.057 || 1.78 || 52.9 | ||
|- | |- | ||
| Blank 3 || --- || | | g29 +dox || 0.987 || 1.77 || 49.4 | ||
|- | |||
| g29 -dox || 0.856 || 1.82 || 42.8 | |||
|- | |||
| +dox || 1.300 || 1.77 || 65.0 | |||
|- | |||
| -dox || 1.169 || 1.77 || 58.5 | |||
|- | |||
| Blank 1+3 || --- || --- || ~21.0 | |||
|} | |} | ||
Line 112: | Line 120: | ||
* 200 ng clean PCR, 1x HF Phusion buffer, in final volume of 15 μL | * 200 ng clean PCR, 1x HF Phusion buffer, in final volume of 15 μL | ||
# 200 ng CRISPR "1" (Zhang approach) | # 200 ng CRISPR "1" (Zhang approach) | ||
# 200 ng Blank (wild type control) | # 200 ng Blank (wild type control) |
Revision as of 06:36, 8 August 2014
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08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
SURVEYOR assay
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