Haynes Lab:Notebook/CRISPR Editing/2014/08/07: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 10: | Line 10: | ||
* CRISPR on chromatin: transfections of g21, g22, g27 into Gal4EED/luc cells | * CRISPR on chromatin: transfections of g21, g22, g27 into Gal4EED/luc cells | ||
* Luc-mCh swap: building the donor sequence | * Luc-mCh swap: building the donor sequence | ||
* SURVEYOR for C-on-C and mCherry | |||
Line 33: | Line 34: | ||
** Add a double stop (TAATAG) onto the end of mCherry | ** Add a double stop (TAATAG) onto the end of mCherry | ||
** Add 130 bp of homology with TK-luciferase to each end | ** Add 130 bp of homology with TK-luciferase to each end | ||
---- | |||
'''SURVEYOR assay for C-on-C and mCherry''' | |||
Revision as of 09:01, 7 August 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
08/07/2014
CRISPR on chromatin: transfections
Luc-mCh swap: building the donor sequence
SURVEYOR assay for C-on-C and mCherry
|