Haynes Lab:Notebook/CRISPR Editing/2014/08/07

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(Autocreate 2014/08/07 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
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* CRISPR on chromatin: transfections of g21, g22, g27 into Gal4EED/luc cells
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* Luc-mCh swap
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----
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'''CRISPR on chromatin: transfections'''<br>
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# gRNA21/Cas9, dox+
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# gRNA22/Cas9, dox+
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# gRNA27/Cas9, dox+
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# gRNA21/Cas9, dox-
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# gRNA22/Cas9, dox-
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# gRNA27/Cas9, dox-
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* On 8/05/14, cells were grown in 4 mL P/S-free medium to ~80% confluence in a 6-well plate, 3 with 1 μg/mL dox, 3 without dox

Revision as of 11:30, 7 August 2014

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08/07/2014

  • CRISPR on chromatin: transfections of g21, g22, g27 into Gal4EED/luc cells
  • Luc-mCh swap



CRISPR on chromatin: transfections

  1. gRNA21/Cas9, dox+
  2. gRNA22/Cas9, dox+
  3. gRNA27/Cas9, dox+
  4. gRNA21/Cas9, dox-
  5. gRNA22/Cas9, dox-
  6. gRNA27/Cas9, dox-
  • On 8/05/14, cells were grown in 4 mL P/S-free medium to ~80% confluence in a 6-well plate, 3 with 1 μg/mL dox, 3 without dox



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