Haynes Lab:Notebook/CRISPR Editing/2014/08/06: Difference between revisions

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{|
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''OD 260'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/uL'''
| align="center" style="background:#f0f0f0;"|'''Average ng/uL'''
|-
| Genomic DNA 7 ()||1.523||1.9||76.1||76.0
|-
| Genomic DNA 7 ()||1.514||1.88||75.7||
|-
| Genomic DNA 7 ()||1.526||1.89||76.3||
|-
| Genomic DNA 8 (gRNA2d)||0.315||1.79||15.7||15.6
|-
| Genomic DNA 8 (gRNA2d)||0.321||1.74||16.1||
|-
| Genomic DNA 8 (gRNA2d)||0.302||1.75||15.1||
|-
| Genomic DNA 9 (gRNA4d)||2.092||1.94||104.6||104.4
|-
| Genomic DNA 9 (gRNA4d)||2.083||1.94||104.2||
|-
| Genomic DNA 9 (gRNA4d)||2.087||1.93||104.4||
|-
| Genomic DNA 10 (gRNA-9)||1.889||1.94||94.5||94.0
|-
| Genomic DNA 10 (gRNA-9)||1.882||1.93||94.1||
|-
| Genomic DNA 10 (gRNA-9)||1.87||1.94||93.5||
|-
| Genomic DNA 1||0.772||1.93||38.6||39.0
|-
| Genomic DNA 1||0.782||1.89||39.1||
|-
| Genomic DNA 1||0.784||1.83||39.2||
|-
| Genomic DNA 2||2.17||1.96||108.5||108.2
|-
| Genomic DNA 2||2.178||1.95||108.9||
|-
| Genomic DNA 2||2.146||1.95||107.3||
|-
| Genomic DNA 3||1.016||1.89||50.8||50.6
|-
| Genomic DNA 3||1.02||1.9||51||
|-
| Genomic DNA 3||1.001||1.94||50.1||
|-
| Genomic DNA 4||1.398||1.91||69.9||68.7
|-
| Genomic DNA 4||1.35||1.93||67.5||
|-
| Genomic DNA 4||1.376||1.9||68.8||
|-
| Genomic DNA 5||4.144||1.99||207.2||206.7
|-
| Genomic DNA 5||4.179||2||209||
|-
| Genomic DNA 5||4.08||2||204||
|-
| Genomic DNA 6||4.907||1.99||245.4||244.0
|-
| Genomic DNA 6||4.9||1.98||245||
|-
| Genomic DNA 6||4.829||1.99||241.5||
|}




Revision as of 10:49, 6 August 2014

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08/06/2014

  • Genomic DNA extractions


Genomic DNA extractions

Samples

  1. HEK gRNA20/Cas9, +dox cells
  2. HEK gRNA20/Cas9, -dox cells
  3. HEK gRNA29/Cas9, +dox cells
  4. HEK gRNA29/Cas9, -dox cells
  5. HEK mock, +dox cells
  6. HEK mock, -dox cells
  7. U2OS 1d
  8. U2OS 2d
  9. U2OS 4d
  10. U2OS mock
  • HEK = Gal4EED/luc cells
  • U2OS = KAH154 CRISPR cells


Sample OD 260 260/280 ng/uL Average ng/uL
Genomic DNA 7 () 1.523 1.9 76.1 76.0
Genomic DNA 7 () 1.514 1.88 75.7
Genomic DNA 7 () 1.526 1.89 76.3
Genomic DNA 8 (gRNA2d) 0.315 1.79 15.7 15.6
Genomic DNA 8 (gRNA2d) 0.321 1.74 16.1
Genomic DNA 8 (gRNA2d) 0.302 1.75 15.1
Genomic DNA 9 (gRNA4d) 2.092 1.94 104.6 104.4
Genomic DNA 9 (gRNA4d) 2.083 1.94 104.2
Genomic DNA 9 (gRNA4d) 2.087 1.93 104.4
Genomic DNA 10 (gRNA-9) 1.889 1.94 94.5 94.0
Genomic DNA 10 (gRNA-9) 1.882 1.93 94.1
Genomic DNA 10 (gRNA-9) 1.87 1.94 93.5
Genomic DNA 1 0.772 1.93 38.6 39.0
Genomic DNA 1 0.782 1.89 39.1
Genomic DNA 1 0.784 1.83 39.2
Genomic DNA 2 2.17 1.96 108.5 108.2
Genomic DNA 2 2.178 1.95 108.9
Genomic DNA 2 2.146 1.95 107.3
Genomic DNA 3 1.016 1.89 50.8 50.6
Genomic DNA 3 1.02 1.9 51
Genomic DNA 3 1.001 1.94 50.1
Genomic DNA 4 1.398 1.91 69.9 68.7
Genomic DNA 4 1.35 1.93 67.5
Genomic DNA 4 1.376 1.9 68.8
Genomic DNA 5 4.144 1.99 207.2 206.7
Genomic DNA 5 4.179 2 209
Genomic DNA 5 4.08 2 204
Genomic DNA 6 4.907 1.99 245.4 244.0
Genomic DNA 6 4.9 1.98 245
Genomic DNA 6 4.829 1.99 241.5



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