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08/06/2014
Genomic DNA extractions
Samples
- HEK gRNA20/Cas9, +dox cells
- HEK gRNA20/Cas9, -dox cells
- HEK gRNA29/Cas9, +dox cells
- HEK gRNA29/Cas9, -dox cells
- HEK mock, +dox cells
- HEK mock, -dox cells
- U2OS 1d
- U2OS 2d
- U2OS 4d
- U2OS mock
- HEK = Gal4EED/luc cells
- U2OS = KAH154 CRISPR cells
| Sample
|
OD 260
|
260/280
|
ng/uL
|
Average ng/uL
|
| Genomic DNA 7 (gRNA1d) |
1.523 |
1.9 |
76.1 |
76.0
|
| Genomic DNA 7 (gRNA1d) |
1.514 |
1.88 |
75.7 |
|
| Genomic DNA 7 (gRNA1d) |
1.526 |
1.89 |
76.3 |
|
| Genomic DNA 8 (gRNA2d) |
0.315 |
1.79 |
15.7 |
15.6
|
| Genomic DNA 8 (gRNA2d) |
0.321 |
1.74 |
16.1 |
|
| Genomic DNA 8 (gRNA2d) |
0.302 |
1.75 |
15.1 |
|
| Genomic DNA 9 (gRNA4d) |
2.092 |
1.94 |
104.6 |
104.4
|
| Genomic DNA 9 (gRNA4d) |
2.083 |
1.94 |
104.2 |
|
| Genomic DNA 9 (gRNA4d) |
2.087 |
1.93 |
104.4 |
|
| Genomic DNA 10 (-g) |
1.889 |
1.94 |
94.5 |
94.0
|
| Genomic DNA 10 (-g) |
1.882 |
1.93 |
94.1 |
|
| Genomic DNA 10 (-g) |
1.87 |
1.94 |
93.5 |
|
| Genomic DNA 1 |
0.772 |
1.93 |
38.6 |
39.0
|
| Genomic DNA 1 |
0.782 |
1.89 |
39.1 |
|
| Genomic DNA 1 |
0.784 |
1.83 |
39.2 |
|
| Genomic DNA 2 |
2.17 |
1.96 |
108.5 |
108.2
|
| Genomic DNA 2 |
2.178 |
1.95 |
108.9 |
|
| Genomic DNA 2 |
2.146 |
1.95 |
107.3 |
|
| Genomic DNA 3 |
1.016 |
1.89 |
50.8 |
50.6
|
| Genomic DNA 3 |
1.02 |
1.9 |
51 |
|
| Genomic DNA 3 |
1.001 |
1.94 |
50.1 |
|
| Genomic DNA 4 |
1.398 |
1.91 |
69.9 |
68.7
|
| Genomic DNA 4 |
1.35 |
1.93 |
67.5 |
|
| Genomic DNA 4 |
1.376 |
1.9 |
68.8 |
|
| Genomic DNA 5 |
4.144 |
1.99 |
207.2 |
206.7
|
| Genomic DNA 5 |
4.179 |
2 |
209 |
|
| Genomic DNA 5 |
4.08 |
2 |
204 |
|
| Genomic DNA 6 |
4.907 |
1.99 |
245.4 |
244.0
|
| Genomic DNA 6 |
4.9 |
1.98 |
245 |
|
| Genomic DNA 6 |
4.829 |
1.99 |
241.5 |
|
qPCR Master Reaction list
| Reaction
|
Templates
|
Primers (Targets)
|
Amplicon
|
Method
|
| 1 |
g20 +dox |
g020US-F/ g020US-R |
152 |
CNV
|
| 2 |
g20 +dox |
GAPDH B2 |
|
CNV
|
| 3 |
g20 -dox |
g020US-F/ g020US-R |
152 |
CNV
|
| 4 |
g20 -dox |
GAPDH B2 |
|
CNV
|
| 5 |
g29 +dox |
g029US-F/ g029US-R |
141 |
CNV
|
| 6 |
g29 +dox |
GAPDH B2 |
|
CNV
|
| 7 |
g29 -dox |
g029US-F/ g029US-R |
141 |
CNV
|
| 8 |
g29 -dox |
GAPDH B2 |
|
CNV
|
| 9 |
+dox |
g020US-F/ g020US-R |
152 |
CNV
|
| 10 |
+dox |
g029US-F/ g029US-R |
141 |
CNV
|
| 11 |
+dox |
GAPDH B2 |
|
CNV
|
| 12 |
-dox |
g020US-F/ g020US-R |
152 |
CNV
|
| 13 |
-dox |
g029US-F/ g029US-R |
141 |
CNV
|
| 14 |
-dox |
GAPDH B2 |
|
CNV
|
| 15 |
1d |
q2F / q3R |
187 |
CNV
|
| 16 |
1d |
GAPDH B2 |
|
CNV
|
| 17 |
2d |
q1F / q1R |
142 |
CNV
|
| 18 |
2d |
GAPDH B2 |
|
CNV
|
| 19 |
4d |
q2F / P2R |
140 |
CNV
|
| 20 |
4d |
GAPDH B2 |
|
CNV
|
| 21 |
mock |
q2F / q3R |
187 |
CNV
|
| 22 |
mock |
q1F / q1R |
142 |
CNV
|
| 23 |
mock |
q2F / P2R |
140 |
CNV
|
| 24 |
mock |
GAPDH B2 |
|
CNV
|
|
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