Haynes Lab:Notebook/CRISPR Editing/2014/08/05: Difference between revisions
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Line 72: | Line 72: | ||
# 200 ng CRISPR "1" (Zhang approach) | # 200 ng CRISPR "1" (Zhang approach) | ||
# 200 ng Blank (wild type control) | # 200 ng Blank (wild type control) | ||
* Temperature program | * Temperature program | ||
Line 79: | Line 80: | ||
** 85ºC -- (‐0.3 ºC/s) --> 75ºC | ** 85ºC -- (‐0.3 ºC/s) --> 75ºC | ||
** 75ºC/ 1 min | ** 75ºC/ 1 min | ||
** 75ºC -- (‐0.3 ºC/s) --> 65ºC | |||
** 65ºC/ 1 min | |||
** 65ºC -- (‐0.3 ºC/s) --> 55ºC | |||
** 55ºC/ 1 min | |||
** 55ºC -- (‐0.3 ºC/s) --> 45ºC | |||
** 45ºC/ 1 min | |||
** 45ºC -- (‐0.3 ºC/s) --> 35ºC | |||
** 35ºC/ 1 min | |||
** 35ºC -- (‐0.3 ºC/s) --> 25 ºC | |||
25 ºC 1 min | ** 25ºC/ 1 min | ||
** 4 ºC/ hold ∞ | |||
Revision as of 11:31, 5 August 2014
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08/05/14
SURVEYOR assay PCR primers [Rene, please add this info]
qPCR melt curve optimization - mCherry CRISPR 1
Optimization parameters
Notes:
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