Haynes Lab:Notebook/CRISPR Editing/2014/08/04: Difference between revisions
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CRISPR 1 - CNV melt | CRISPR 1 - CNV melt | ||
* Primer pair 1 (q2F_q3R) | * Primer pair 1 (q2F_q3R), Temp = 86.60 | ||
** Untreated control Peak_q2F_q3R / Peak_GAPDH = | ** Untreated control Peak_q2F_q3R / Peak_GAPDH = 5273.00 / 2959.00 = 1.78 | ||
** CRISPR treated Peak_q2F_q3R / Peak_GAPDH = | ** CRISPR treated Peak_q2F_q3R / Peak_GAPDH = 5124.00 / 2902.00 = 1.77 | ||
** Conclusion: | ** Conclusion: '''Hypothesis is supported, with reservation (small value, no replicates)''' | ||
** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ||
CRISPR 1 - CNV melt | CRISPR 1 - CNV melt | ||
* Primer pair 2 (P3F_q3R) | * Primer pair 2 (P3F_q3R) | ||
** Untreated control Peak_P3F_q3R / Peak_GAPDH = | ** Untreated control Peak_P3F_q3R / Peak_GAPDH = 4922.00 / 2959.00 = 1.66 | ||
** CRISPR treated Peak_P3F_q3R / Peak_GAPDH = | ** CRISPR treated Peak_P3F_q3R / Peak_GAPDH = 4693.00 / 2902.00 = 1.62 | ||
** Conclusion: | ** Conclusion: | ||
** Notes: ''some signal from nTc, not-so-good primer pair'' | ** Notes: ''some signal from nTc, not-so-good primer pair'' | ||
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| Experiment || Method || Norm Expt. value || Norm control || Conclusion | | Experiment || Method || Norm Expt. value || Norm control || Conclusion | ||
|- | |- | ||
| "1" - gRNA1/Cas9, primer pair 1 || CNV melt || | | "1" - gRNA1/Cas9, primer pair 1 || CNV melt || 1.77 || 1.78 || Hypothesis 1 supported* | ||
|- | |- | ||
| "1" - gRNA1/Cas9, primer pair 2 || CNV melt || | | "1" - gRNA1/Cas9, primer pair 2 || CNV melt || 1.62 || 1.66 || Hypothesis 1 supported* | ||
|- | |- | ||
| "2" - gRNA2/Cas9 || CNV melt || 0. | | "2" - gRNA2/Cas9 || CNV melt || 0.96 || 0.92 || Hypothesis 1 rejected | ||
|- | |- | ||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || CNV melt || 1.16 || 0.92 || Hypothesis 1 rejected | | "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || CNV melt || 1.16 || 0.92 || Hypothesis 1 rejected | ||
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| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n primer pair 1 || US-specific || 11.71 || 7.73 || Hypothesis 2 rejected | | "4" - gRNA4T/Cas9n, gRNA4B/Cas9n primer pair 1 || US-specific || 11.71 || 7.73 || Hypothesis 2 rejected | ||
|- | |- | ||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n preimer pair 2 || US-specific || 284.05 || 533.74 || Hypothesis 2 supported | | "4" - gRNA4T/Cas9n, gRNA4B/Cas9n preimer pair 2 || US-specific || 284.05 || 533.74 || Hypothesis 2 supported* | ||
|} | |} | ||
Note: *Hypothesis supported, with reservation | |||
Revision as of 19:45, 5 August 2014
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08/04/14
mCherry CRISPR qPCR
Master reaction list
Notes:
CRISPR 1 - CNV melt
CRISPR 1 - CNV melt
CRISPR 2 - CNV melt
CRISPR 4 - CNV melt
CRISPR 4 - US-specific
CRISPR 4 - US-specific
Note: *Hypothesis supported, with reservation
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