Haynes Lab:Notebook/CRISPR Editing/2014/08/04: Difference between revisions
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** Untreated control 2^(Cq_GAPDH - Cq_P3F_q3R) = 2^(36.43 - 27.37) = 533.74 | ** Untreated control 2^(Cq_GAPDH - Cq_P3F_q3R) = 2^(36.43 - 27.37) = 533.74 | ||
** CRISPR treated 2^(Cq_GAPDH - Cq_P3F_q3R) = 2^(35.51 - 27.36) = 284.05 | ** CRISPR treated 2^(Cq_GAPDH - Cq_P3F_q3R) = 2^(35.51 - 27.36) = 284.05 | ||
** Normalized CRISPR treated value = 0.53 | |||
** Conclusion: '''Hypothesis 2 is supported, with reservation''' | ** Conclusion: '''Hypothesis 2 is supported, with reservation''' | ||
** Notes: ''some signal from nTc (Cq ~42), not-so-good primer pair'' | ** Notes: ''some signal from nTc (Cq ~42), not-so-good primer pair'' | ||
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| "1" - gRNA1/Cas9, primer pair 2 || CNV melt || ### || ### || ### | | "1" - gRNA1/Cas9, primer pair 2 || CNV melt || ### || ### || ### | ||
|- | |- | ||
| "2" - gRNA2/Cas9 || CNV melt || | | "2" - gRNA2/Cas9 || CNV melt || 0.954 || 0.92 || Hypothesis 1 rejected | ||
|- | |- | ||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || CNV melt || | | "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || CNV melt || 1.16 || 0.92 || Hypothesis 1 rejected | ||
|- | |- | ||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || US-specific || | | "4" - gRNA4T/Cas9n, gRNA4B/Cas9n primer pair 1 || US-specific || 11.71 || 7.73 || Hypothesis 2 rejected | ||
|- | |- | ||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || US-specific || | | "4" - gRNA4T/Cas9n, gRNA4B/Cas9n preimer pair 2 || US-specific || 284.05 || 533.74 || Hypothesis 2 supported | ||
|} | |} | ||
Revision as of 13:41, 5 August 2014
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08/04/14
mCherry CRISPR qPCR
Master reaction list
Notes:
CRISPR 1 - CNV melt
CRISPR 1 - CNV melt
CRISPR 2 - CNV melt
CRISPR 4 - CNV melt
CRISPR 4 - US-specific
CRISPR 4 - US-specific
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