Haynes Lab:Notebook/CRISPR Editing/2014/08/04: Difference between revisions
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CRISPR 2 - CNV melt | CRISPR 2 - CNV melt | ||
* Primer pair (q1F_q1R) | * Primer pair (q1F_q1R), Temp = 87.53 | ||
** Untreated control Peak_q1F_q1R / Peak_GAPDH = | ** Untreated control Peak_q1F_q1R / Peak_GAPDH = 2361.38 / 2481.73 = 0.952 | ||
** CRISPR treated Peak_q1F_q1R / Peak_GAPDH = | ** CRISPR treated Peak_q1F_q1R / Peak_GAPDH = 2292.12 / 2401.67 = 0.954 | ||
** Conclusion: | ** Conclusion: Hypothesis 1 rejected | ||
** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ||
CRISPR 4 - CNV melt | CRISPR 4 - CNV melt | ||
* Primer pair (q2F_P2R) | * Primer pair (q2F_P2R), Temp = 87.20 | ||
** Untreated control Peak_q2F_P2R / Peak_GAPDH = | ** Untreated control Peak_q2F_P2R / Peak_GAPDH = 2270.05 / 2481.73 = 0.92 | ||
** CRISPR treated Peak_q2F_P2R / Peak_GAPDH = | ** CRISPR treated Peak_q2F_P2R / Peak_GAPDH = 2970.81 / 2548.25 = 1.16 | ||
** Conclusion: | ** Conclusion: Hypothesis 1 rejected | ||
** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ** Notes: no signal from nTc, so good primer pair with no primer dimer signal | ||
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|- | |- | ||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || CNV melt || ### || ### || ### | | "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || CNV melt || ### || ### || ### | ||
|- | |||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || US-specific || ### || ### || ### | |||
|- | |||
| "4" - gRNA4T/Cas9n, gRNA4B/Cas9n || US-specific || ### || ### || ### | |||
|} | |} | ||
Revision as of 12:37, 5 August 2014
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08/04/14
mCherry CRISPR qPCR
Master reaction list
Notes:
CRISPR 1 - CNV melt
CRISPR 1 - CNV melt
CRISPR 2 - CNV melt
CRISPR 4 - CNV melt
CRISPR 4 - US-specific
CRISPR 4 - US-specific
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