Haynes Lab:Notebook/CRISPR Editing/2014/08/04: Difference between revisions
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==08/04/14== | ==08/04/14== | ||
* mCherry CRISPR qPCR | * mCherry CRISPR qPCR | ||
* | * Gal4EED/luc CRISPR - change growth medium to pen-strep-free, with 1 μg/mL dox for dox+ cells | ||
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'''Results''' | '''Results''' | ||
* Hypothesis 1 - CNV melt: CRISPR editing will result in a lower loading-normalized peak height value, compared to untreated cells | |||
* Hypothesis 2 - US-specific amplification: CRISPR editing will result in a lower loading-normalized Cq value, compared to untreated cells | |||
CRISPR 1 - CNV melt | CRISPR 1 - CNV melt | ||
* Primer pair 1 (q2F_q3R) | * Primer pair 1 (q2F_q3R) | ||
** Untreated control Peak_q2F_q3R / Peak_GAPDH = | ** Untreated control Peak_q2F_q3R / Peak_GAPDH = | ||
** | ** CRISPR treated Peak_q2F_q3R / Peak_GAPDH = | ||
** Conclusion: | ** Conclusion: | ||
** Notes: no signal from nTc, so good primer pair with nor primer dimer signal | ** Notes: no signal from nTc, so good primer pair with nor primer dimer signal | ||
CRISPR 2 - CNV melt | |||
* Primer pair 1 (q2F_q3R) | * Primer pair 1 (q2F_q3R) | ||
** Untreated control Peak_q2F_q3R / Peak_GAPDH = | ** Untreated control Peak_q2F_q3R / Peak_GAPDH = | ||
Revision as of 06:21, 5 August 2014
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08/04/14
mCherry CRISPR qPCR
Master reaction list
CRISPR 1 - CNV melt
CRISPR 2 - CNV melt
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