Haynes Lab:Notebook/CRISPR Editing/2014/08/04: Difference between revisions
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==08/04/14== | ==08/04/14== | ||
* mCherry CRISPR qPCR | * mCherry CRISPR qPCR | ||
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| 28||nTc||GAPDH B2||CNV melt | | 28||nTc||GAPDH B2||CNV melt | ||
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'''Results''' | |||
CRISPR 1 - CNV melt | |||
* Primer pair 1 (q2F_q3R) | |||
** Untreated control Peak_q2F_q3R / Peak_GAPDH = | |||
** Untreated control Peak_q2F_q3R / Peak_GAPDH = | |||
** Conclusion: | |||
** Notes: no signal from nTc, so good primer pair with nor primer dimer signal | |||
Experiment 1 - CNV melt | |||
* Primer pair 1 (q2F_q3R) | |||
** Untreated control Peak_q2F_q3R / Peak_GAPDH = | |||
** Untreated control Peak_q2F_q3R / Peak_GAPDH = | |||
** Conclusion: | |||
Revision as of 06:16, 5 August 2014
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08/04/14
mCherry CRISPR qPCR
Master reaction list
CRISPR 1 - CNV melt
Experiment 1 - CNV melt
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