Haynes Lab:Notebook/CRISPR Editing/2014/08/03: Difference between revisions
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(Autocreate 2014/08/03 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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* Gal4EED/luc transfection | |||
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'''Gal4EED/luc transfection''' | |||
* Lipofectamine LTX transfection of cells, 6-well plate format | |||
* Gal4EED/luc +dox from T-25 tryspinized and plated in wells 1-3 (4 mL medium) | |||
* Gal4EED/luc (no dox) from T-25 tryspinized and plated in wells 4-6 (4 mL medium) | |||
** Growth medium added back to original flask to grow up remaining cells as back-up | |||
* Transfection carried out as described in the CSH Syn Bio 2014 manual (2000 ng DNA/ 600 μL transfection mix/ 4 mL cell sample) | |||
Revision as of 10:32, 4 August 2014
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08/03/2014
Gal4EED/luc transfection
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