Haynes Lab:Notebook/CRISPR Editing/2014/07/11: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/07/11 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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==07//2014== | ==07/11/2014== | ||
Undergrad Cameron Gardner mini prepped cells picked from plates 5 and 14. The cells picked from plate 13 didn't grow. <br> | |||
Cameron cut with BbsI and EcoRI. <br> | |||
''Uncut and single cut plasmid should give two bands because there should be one-two BbsI bands bands 5.2kb and 3.2kb. Double cut backbone and correct ligations should give one 8kb band because there should not be a BbsI band.'' | |||
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[[Image:14.07.11 gel.png|700px|Digest gel]] | |||
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Revision as of 14:34, 15 July 2014
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07/11/2014Undergrad Cameron Gardner mini prepped cells picked from plates 5 and 14. The cells picked from plate 13 didn't grow. | |
