Project Description/Abstract
Team Chimeric Reporter
- Hyder Hussain: Topo target plasmid design and building
- Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
- Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
- Ryan Muller: Inducible Topo chimeric protein production
- Ethan Ward: Inducible Topo chimeric protein production
Team Cell Surface Reporter
- Nisarg Patel: Split beta gal assay development for the magainin reporter
- Ellen Qin: Magainin fusion protein BioBricking
Support
- Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
- Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
- Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions
BioBricks
Magainin Reporter
- Magainin
- Magainin + 2xGS Linker + His-Tag
- Bgal fragment (a-1)
- Bgal fragment (a-4)
- Bgal fragment (w)
- Bgal fragment (1-w)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
- His-Tag + 10aa GS Linker + Bgal fragment (a-1)
- His-Tag + 10aa GS Linker + Bgal fragment (a-4)
- 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
- 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
Resources
- A Plasmid Editor (ApE): freeware for viewing, editing, annotating, translating, and finding restriction sites in DNA sequences
- Oligator: tool for creating BioBricks from oligo assembly
- Protein Data Bank (PDB): search for, view, and download full atomic structure data for 3D structures of proteins and other molecules
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