Haynes Lab:Notebook/ASU iGEM

From OpenWetWare

< Haynes Lab:Notebook(Difference between revisions)
Jump to: navigation, search
(Resources)
Current revision (02:13, 17 August 2012) (view source)
 
(10 intermediate revisions not shown.)
Line 53: Line 53:
-
==Resources==
+
==Science Resources==
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ A Plasmid Editor (ApE)]: freeware for viewing, editing, annotating, translating, and finding restriction sites in DNA sequences
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ A Plasmid Editor (ApE)]: freeware for viewing, editing, annotating, translating, and finding restriction sites in DNA sequences
* [http://gcat.davidson.edu/igem10/ Oligator]: tool for creating BioBricks using oligo assembly
* [http://gcat.davidson.edu/igem10/ Oligator]: tool for creating BioBricks using oligo assembly
-
* [http://www.rcsb.org/pdb/home/home.do Protein Data Bank (PDB)]: search for, view, and download full atomic structure data for 3D structures of proteins and other molecules. Get tips on editing Jmol 3D images at the [http://openwetware.org/wiki/Haynes:Jmol_Guide Haynes Lab OWW site].
+
* [http://www.rcsb.org/pdb/home/home.do Protein Data Bank (PDB)]: search for, view, and download full atomic coordinate data for 3D structures of proteins and other molecules. Get tips on editing Jmol 3D images at the [http://openwetware.org/wiki/Haynes:Jmol_Guide Haynes Lab OWW site].
* [http://openwetware.org/wiki/Haynes:Protocols Protocols - Haynes Lab]: some protocols from the Haynes Lab
* [http://openwetware.org/wiki/Haynes:Protocols Protocols - Haynes Lab]: some protocols from the Haynes Lab
 +
 +
==General Lab Links==
 +
* [https://docs.google.com/spreadsheet/ccc?key=0AghpgNgJLx-ldGtGSUhRRjFRQ0VCdlo4WXdyNlVwWVE Ordering Spreadsheet]
 +
 +
==Safety==
 +
* [https://cfo.asu.edu/ehs Environmental Health & Safety at ASU]
 +
* [http://ehsaweb.asu.edu/ Individual Training Record]: To check your ASU individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.
__NOTOC__
__NOTOC__

Current revision

ASU iGEM 2012

Search this Project

Customize your entry pages

Project Description/Abstract

Team Chimeric Reporter

  • Hyder Hussain: Topo target plasmid design and building
  • Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Ryan Muller: Inducible Topo chimeric protein production
  • Ethan Ward: Inducible Topo chimeric protein production


Team Cell Surface Reporter

  • Nisarg Patel: Split beta gal assay development for the magainin reporter
  • Ellen Qin: Magainin fusion protein BioBricking


Support

  • Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions


BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)


Science Resources

General Lab Links

Safety


Personal tools