Haynes Lab:Notebook/ASU iGEM

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* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ A Plasmid Editor (ApE)]: freeware for viewing, editing, annotating, translating, and finding restriction sites in DNA sequences
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ A Plasmid Editor (ApE)]: freeware for viewing, editing, annotating, translating, and finding restriction sites in DNA sequences
* [http://gcat.davidson.edu/igem10/ Oligator]: tool for creating BioBricks using oligo assembly
* [http://gcat.davidson.edu/igem10/ Oligator]: tool for creating BioBricks using oligo assembly
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* [http://www.rcsb.org/pdb/home/home.do Protein Data Bank (PDB)]: search for, view, and download full atomic structure data for 3D structures of proteins and other molecules. Get tips on editing Jmol 3D images at the [http://openwetware.org/wiki/Haynes:Jmol_Guide Haynes Lab OWW site].
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* [http://www.rcsb.org/pdb/home/home.do Protein Data Bank (PDB)]: search for, view, and download full atomic coordinate data for 3D structures of proteins and other molecules. Get tips on editing Jmol 3D images at the [http://openwetware.org/wiki/Haynes:Jmol_Guide Haynes Lab OWW site].
* [http://openwetware.org/wiki/Haynes:Protocols Protocols - Haynes Lab]: some protocols from the Haynes Lab
* [http://openwetware.org/wiki/Haynes:Protocols Protocols - Haynes Lab]: some protocols from the Haynes Lab
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Revision as of 01:29, 5 July 2012

ASU iGEM 2012

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Project Description/Abstract

Team Chimeric Reporter

  • Hyder Hussain: Topo target plasmid design and building
  • Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Ryan Muller: Inducible Topo chimeric protein production
  • Ethan Ward: Inducible Topo chimeric protein production


Team Cell Surface Reporter

  • Nisarg Patel: Split beta gal assay development for the magainin reporter
  • Ellen Qin: Magainin fusion protein BioBricking


Support

  • Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions


BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)


Resources


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