Haynes Lab:Notebook/ASU iGEM

ASU iGEM 2012

Project Description/Abstract

Team Chimeric Reporter

• Hyder Hussain: Topo target plasmid design and building
• Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
• Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
• Ryan Muller: Inducible Topo chimeric protein production
• Ethan Ward: Inducible Topo chimeric protein production

Team Cell Surface Reporter

• Nisarg Patel: Split beta gal assay development for the magainin reporter
• Ellen Qin: Magainin fusion protein BioBricking

Support

• Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
• Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
• Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions

BioBricks

Magainin Reporter

• Magainin
• Magainin + 2xGS Linker + His-Tag
• Bgal fragment (a-1)
• Bgal fragment (a-4)
• Bgal fragment (w)
• Bgal fragment (1-w)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
• His-Tag + 10aa GS Linker + Bgal fragment (a-1)
• His-Tag + 10aa GS Linker + Bgal fragment (a-4)
• 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
• 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)

Resources

• A Plasmid Editor (ApE): freeware for viewing, editing, annotating, translating, and finding restriction sites in DNA sequences
• Oligator: tool for creating BioBricks from oligo assembly
• Protein Data Bank (PDB): search for, view, and download full atomic structure data for 3D structures of proteins and other molecules