Haynes Lab:Notebook/ASU iGEM

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==Project Description/Abstract==
==Project Description/Abstract==
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[[#THAT| '''Team Chimeric Reporter:''']]<br>
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'''Team Chimeric Reporter'''<br>
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* Hyder Hussain
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* Hyder Hussain: Topo target plasmid design and building
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* Abhinav Markus
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* Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
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* Ryan Muller
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* Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
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* Ethan Ward
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* Ryan Muller: Inducible Topo chimeric protein production
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[[#THIS| '''Team Cell Surface Reporter:''']]<br>
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* Ethan Ward: Inducible Topo chimeric protein production
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* Nisarg Patel
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* Ryan Muller
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* Rohit Rajan
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'''Team Cell Surface Reporter'''<br>
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* Ellen Qin
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* Nisarg Patel: Split beta gal assay development for the magainin reporter
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* Melinda Jenner
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* Ellen Qin: Magainin fusion protein BioBricking
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 +
 
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'''Support'''<br>
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* Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
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* Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
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* Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions
==BioBricks==
==BioBricks==

Revision as of 21:39, 3 July 2012

ASU iGEM 2012

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Project Description/Abstract

Team Chimeric Reporter

  • Hyder Hussain: Topo target plasmid design and building
  • Abhinav Markus: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Maddie Sands: (new) Split beta gal assay development for the chimeric reporter (biotinylation proof of concept model)
  • Ryan Muller: Inducible Topo chimeric protein production
  • Ethan Ward: Inducible Topo chimeric protein production


Team Cell Surface Reporter

  • Nisarg Patel: Split beta gal assay development for the magainin reporter
  • Ellen Qin: Magainin fusion protein BioBricking


Support

  • Rohit Rajan: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Melinda Jenner: Buffers, media (and quality control), preparation of BioBrick registry submissions
  • Amanda Ispas: Buffers, media (and quality control), preparation of BioBrick registry submissions

BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)

Other Notes
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