Haynes Lab:Notebook/ASU iGEM

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 46: Line 46:
*14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
*14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
-
==Notes==
+
==Other Notes==
-
 
+
-
 
+
-
 
+
-
 
+
-
===6-15-12===
+
-
*Competent cell prep
+
-
:*Centrifuged falcon test tubes containing liquid colonies
+
-
:*Resuspended in CaCl<sub>2</sub> buffer solution and incubated for 15 mins
+
-
:*Centrifuged and resuspended in CaCl<sub>2</sub> glycerol buffer solution
+
-
:*Chilled overnight
+

Revision as of 20:32, 3 July 2012

ASU iGEM 2012

Search this Project

Customize your entry pages

Project Description/Abstract

Team Chimeric Reporter:

  • Hyder Hussain
  • Abhinav Markus
  • Ryan Muller
  • Ethan Ward

Team Cell Surface Reporter:

  • Nisarg Patel
  • Ryan Muller
  • Rohit Rajan
  • Ellen Qin
  • Melinda Jenner
  • Joe Barth

BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)

Other Notes

Personal tools