Haynes Lab:Notebook/ASU iGEM

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*14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
*14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
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==Notes==
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==Other Notes==
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===6-15-12===
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*Competent cell prep
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:*Centrifuged falcon test tubes containing liquid colonies
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:*Resuspended in CaCl<sub>2</sub> buffer solution and incubated for 15 mins
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:*Centrifuged and resuspended in CaCl<sub>2</sub> glycerol buffer solution
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:*Chilled overnight
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Revision as of 21:32, 3 July 2012

ASU iGEM 2012

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Project Description/Abstract

Team Chimeric Reporter:

  • Hyder Hussain
  • Abhinav Markus
  • Ryan Muller
  • Ethan Ward

Team Cell Surface Reporter:

  • Nisarg Patel
  • Ryan Muller
  • Rohit Rajan
  • Ellen Qin
  • Melinda Jenner
  • Joe Barth

BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)

Other Notes
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