Haynes Lab:Notebook/ASU iGEM

ASU iGEM 2012

Project Description/Abstract

Team Chimeric Reporter:

• Hyder Hussain
• Abhinav Markus
• Ryan Muller
• Ethan Ward

Team Cell Surface Reporter:

• Nisarg Patel
• Ryan Muller
• Rohit Rajan
• Ellen Qin
• Melinda Jenner
• Joe Barth

BioBricks

Magainin Reporter

• Magainin
• Magainin + 2xGS Linker + His-Tag
• Bgal fragment (a-1)
• Bgal fragment (a-4)
• Bgal fragment (w)
• Bgal fragment (1-w)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
• Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
• His-Tag + 10aa GS Linker + Bgal fragment (a-1)
• His-Tag + 10aa GS Linker + Bgal fragment (a-4)
• 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
• 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)

Notes

6-15-12

• Competent cell prep

• Centrifuged falcon test tubes containing liquid colonies
• Resuspended in CaCl₂ buffer solution and incubated for 15 mins
• Centrifuged and resuspended in CaCl₂ glycerol buffer solution
• Chilled overnight