Haynes Lab:Notebook/ASU iGEM

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:*Centrifuged and resuspended in CaCl<sub>2</sub> glycerol buffer solution
:*Centrifuged and resuspended in CaCl<sub>2</sub> glycerol buffer solution
:*Chilled overnight
:*Chilled overnight
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===6-16-12===
 
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*Competent cell prep
 
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:*Aliquotted 200uL into test tubes
 
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:*Stored in -80C
 

Revision as of 20:31, 3 July 2012

ASU iGEM 2012

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Project Description/Abstract

Team Chimeric Reporter:

  • Hyder Hussain
  • Abhinav Markus
  • Ryan Muller
  • Ethan Ward

Team Cell Surface Reporter:

  • Nisarg Patel
  • Ryan Muller
  • Rohit Rajan
  • Ellen Qin
  • Melinda Jenner
  • Joe Barth

BioBricks

Magainin Reporter

  • Magainin
  • Magainin + 2xGS Linker + His-Tag
  • Bgal fragment (a-1)
  • Bgal fragment (a-4)
  • Bgal fragment (w)
  • Bgal fragment (1-w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
  • Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-1)
  • His-Tag + 10aa GS Linker + Bgal fragment (a-4)
  • 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
  • 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)

Notes

6-15-12

  • Competent cell prep
  • Centrifuged falcon test tubes containing liquid colonies
  • Resuspended in CaCl2 buffer solution and incubated for 15 mins
  • Centrifuged and resuspended in CaCl2 glycerol buffer solution
  • Chilled overnight




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