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| | ::*Colonies observed | | ::*Colonies observed |
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| - | ===6-19-12===
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| - | *Transformation (LSE)
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| - | :*Transformed DNA:
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| - | ::*T7 promoter BBa_I712074
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| - | ::*Constitutive promoter BBa_J23102
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| - | :* Cells: DH5 alpha (donated)
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| - | :* Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
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| - | :* Controls: puc19, no DNA
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| - | :* Plated 2 copies of each (100 ul, 250 ul) on LB amp plates.
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| - | * Made 50 LB Amp plates.
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Revision as of 21:29, 3 July 2012
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 ASU iGEM 2012
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Customize your entry pages 
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Project Description/Abstract
Team Chimeric Reporter:
- Hyder Hussain
- Abhinav Markus
- Ryan Muller
- Ethan Ward
Team Cell Surface Reporter:
- Nisarg Patel
- Ryan Muller
- Rohit Rajan
- Ellen Qin
- Melinda Jenner
- Joe Barth
BioBricks
Magainin Reporter
- Magainin
- Magainin + 2xGS Linker + His-Tag
- Bgal fragment (a-1)
- Bgal fragment (a-4)
- Bgal fragment (w)
- Bgal fragment (1-w)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-1)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (a-4)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (w)
- Magainin + 2aa GS Linker + His-Tag + 10aa GS Linker + Bgal fragment (1-w)
- His-Tag + 10aa GS Linker + Bgal fragment (a-1)
- His-Tag + 10aa GS Linker + Bgal fragment (a-4)
- 13. His-Tag + 10aa GS Linker + Bgal fragment (w)
- 14. His-Tag + 10aa GS Linker + Bgal fragment (1-w)
Notes
6-15-12
- Centrifuged falcon test tubes containing liquid colonies
- Resuspended in CaCl2 buffer solution and incubated for 15 mins
- Centrifuged and resuspended in CaCl2 glycerol buffer solution
- Chilled overnight
6-16-12
- Aliquotted 200uL into test tubes
- Stored in -80C
6-17-12
- Streak plated prepared competent cells on LB no amp plate
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