Haynes Lab: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
(111 intermediate revisions by 10 users not shown)
Line 1: Line 1:
[[Image:Asu_logo_2.jpg]]
<div style="padding: 5px; background-color: #ffc125; width: 1000px; text-align: left">
<div style="padding: 10px; color: #ffffff; background-color: #000; width: 730px">
[[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
<center>
&nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Contact | <font face="trebuchet ms" style="color:#000000"> '''Contact''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel''' </font>]] &nbsp;&nbsp;&nbsp;
[[Haynes:Calendar | <font face="trebuchet ms" style="color:#000000"> '''Calendar''' </font>]]
</div><br>
 
<div style="padding-left: 5px; padding-right: 80px; float: left; text-align: justify; width: 600px; height: 800px">
Our group uses synthetic, systems, and quantitative biology to engineer useful gene and protein-based biological devices and to deepen our understanding of molecular cell biology. We operate biological devices primarily in human/ mammalian cells. Accelerating the pace of therapeutic technologies (such as tissue regeneration and customizable protein-based drugs) via modular design is the grand challenge that shapes our research plans.
<br><br>
 
CURRENT SYNTHETIC BIOMEDICAL ENGINEERING PROJECTS<br>
 
'''Synthetic chromatin for cell differentiation'''
* David Barclay (FURI & SynBERC, BS) - pancreas, Jan Simper (FURI, BS) - iPSC's
* Description: Testing the "PcTF" synthetic chromatin protein/transcription activator described in [http://www.ncbi.nlm.nih.gov/pubmed/21669865 Haynes & Silver 2011] to determine its ability to alter the phenotypes of healthy cells, such as cell fate switching from alpha cells to beta cells in the pancreas to treat diabetes or the growing of programmable "organoids" from and transfection of iPS cells to enhance current measurements of pluripotency.
 
 
'''Editing synthetic genes using CRISPR'''
* René Davis (Biological Design, PhD)
* Description: Characterizing chromatin/CRISPR interactions. Re-engineering synthetic gene circuits in human cells using CRISPR.
 
 
'''Engineering synthetic chromatin transcription factors'''
* Cameron Gardner (FURI, BS)
* Description: Building and testing re-engineered versions of the "PcTF" synthetic chromatin protein/ transcription activator described in [http://www.ncbi.nlm.nih.gov/pubmed/21669865 Haynes & Silver 2011].
 
 
'''Microbial communication with synthetic quorum sensing'''
* René Davis (Biological Design, PhD), Ryan Muller (SOLUR, BS)
* Description: Characterizing cross-talk between decoupled cell-cell communication systems from bacteria.


[[Haynes Lab | <font face="trebuchet ms" style="color:#ffffff"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</div>
[[Haynes Lab | <font face="trebuchet ms" style="color:#ffffff"> '''Contact''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
[[Haynes:Back Door | <font face="trebuchet ms" style="color:#ffffff"> '''Internal''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
'''Lab Notebooks - Active''' {{hide|
[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#ffffff"> '''Lab Members''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/SynBERC_Scholars_2013 Alfonso & Zazu]
[[Haynes:Publications | <font face="trebuchet ms" style="color:#ffffff"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
* [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli Behzad]
[[Haynes:Research | <font face="trebuchet ms" style="color:#ffffff"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/RNA-seq_of_PcTF_Transfected_U2OS_%26_SK-N-SH_cell_lines Ben]
[[Haynes:Talks | <font face="trebuchet ms" style="color:#ffffff"> '''Talks''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs Cameron]
</center>
* [http://openwetware.org/wiki/User:David_Barclay/Notebook David Barclay]
</div><br>
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Heathers_Lab_Notebook Heather]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan Jan]
* [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook Karmella]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi#Pradyumna_Kadambi.2FUndergraduate_Lab_Traning Prad]
* [http://openwetware.org/wiki/User:Rene_M_Davis/Notebooks René - Quorum Sensing]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/CRISPR_Editing René - CRISPR Editing]
* [http://openwetware.org/wiki/User:Brendan_F._Fries/Notebook/Brendan_Fries Brendan]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook:Shared/Cloning_Bootcamp Cloning Bootcamp]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Short_Projects Short Projects]
}}
'''Lab Notebooks - Archived''' {{hide|
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Chimera_Biosensor_%28C.R.SYS%29 Abhi]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Inteins Amanda]
* [http://openwetware.org/wiki/Carly's_Lab_Notebook Carly]
* [http://openwetware.org/wiki/User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern David Dreher]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Heathers_Lab_Notebook Heather]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/James_Alling James]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi#Pradyumna_Kadambi.2FUndergraduate_Lab_Traning Prad]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes Vi]
* [http://openwetware.org/wiki/User:Wesley_J._Houston/Notebook/MAP Wesley]
* [http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM ASU iGEM 2012]
}}
'''[https://benchling.com/hayneslab Benchling]'''<br>
'''[https://trello.com/hayneslab Trello Boards]'''

Revision as of 09:42, 21 January 2015


Our group uses synthetic, systems, and quantitative biology to engineer useful gene and protein-based biological devices and to deepen our understanding of molecular cell biology. We operate biological devices primarily in human/ mammalian cells. Accelerating the pace of therapeutic technologies (such as tissue regeneration and customizable protein-based drugs) via modular design is the grand challenge that shapes our research plans.

CURRENT SYNTHETIC BIOMEDICAL ENGINEERING PROJECTS

Synthetic chromatin for cell differentiation

  • David Barclay (FURI & SynBERC, BS) - pancreas, Jan Simper (FURI, BS) - iPSC's
  • Description: Testing the "PcTF" synthetic chromatin protein/transcription activator described in Haynes & Silver 2011 to determine its ability to alter the phenotypes of healthy cells, such as cell fate switching from alpha cells to beta cells in the pancreas to treat diabetes or the growing of programmable "organoids" from and transfection of iPS cells to enhance current measurements of pluripotency.


Editing synthetic genes using CRISPR

  • René Davis (Biological Design, PhD)
  • Description: Characterizing chromatin/CRISPR interactions. Re-engineering synthetic gene circuits in human cells using CRISPR.


Engineering synthetic chromatin transcription factors

  • Cameron Gardner (FURI, BS)
  • Description: Building and testing re-engineered versions of the "PcTF" synthetic chromatin protein/ transcription activator described in Haynes & Silver 2011.


Microbial communication with synthetic quorum sensing

  • René Davis (Biological Design, PhD), Ryan Muller (SOLUR, BS)
  • Description: Characterizing cross-talk between decoupled cell-cell communication systems from bacteria.

Lab Notebooks - Active

Lab Notebooks - Archived

Benchling
Trello Boards

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab&oldid=852135"