Haynes:WikiTemp NBplasmids

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Plasmid Validation Template page

DNA Concentration Data

Plasmid	OD260	OD260/280	ng/μL
1. Plasmid 1	---	---	---
2. Plasmid 2	---	---	---

Restriction Digest Table

• Checked plasmid minipreps with EcoRI/PstI digests

Reagent	Volume	Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size	15 μL/lane; 1% agarose; Ladder
DNA(plasmid)	3.0 μL
10X buffer	1.5
EcoRI	1.0
PstI	1.0
dH2O	8.5
	15 μL --> 37°C/ 15 min.

DNA Sequencing Samples

• Submitted to DNASU on: 'date'
• Order Number:

1. Plasmid 1 - forward primer 'name'
2. Plasmid 1 - reverse primer 'name'
3. Plasmid 2 - forward primer 'name'
4. Plasmid 2 - reverse primer 'name'