Haynes:WikiTemp NBplasmids

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DNA Concentration Data


Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Check plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.
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