Haynes:WikiTemp NBplasmids: Difference between revisions
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'''DNA Concentration Data''' | '''DNA Concentration Data''' | ||
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| Plasmid || OD260 || OD260/280 || ng/μL | | Plasmid || OD260 || OD260/280 || ng/μL | ||
Revision as of 07:55, 3 September 2013
DNA Concentration Data
| Plasmid | OD260 | OD260/280 | ng/μL |
| 1. Plasmid 1 | --- | --- | --- |
| 2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Check plasmid minipreps with EcoRI/PstI digests
| Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
 15 μL/lane; 1% agarose; Ladder |
| DNA(plasmid) | 3.0 μL | ||
| 10X buffer | 1.5 | ||
| EcoRI | 1.0 | ||
| PstI | 1.0 | ||
| dH2O | 8.5 | ||
| 15 μL --> 37°C/ 15 min. |
DNA Sequencing Samples
- Submitted to DNASU on 'date'
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
