Haynes:WikiTemp NBplasmids

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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home] | [http://openwetware.org/wiki/Haynes:TechResources#Wiki_Editing Wiki Editing]
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=Plasmid Validation Template page=
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'''Restriction Digest Table'''<br>
'''Restriction Digest Table'''<br>
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* Check plasmid minipreps with EcoRI/PstI digests
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* Checked plasmid minipreps with EcoRI/PstI digests
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'''DNA Sequencing Samples'''
'''DNA Sequencing Samples'''
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* Submitted to DNASU on 'date'
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* Submitted to DNASU on: 'date'
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* Order Number:
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
# Plasmid 1 - forward primer 'name'
# Plasmid 1 - forward primer 'name'
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# Plasmid 2 - forward primer 'name'
# Plasmid 2 - forward primer 'name'
# Plasmid 2 - reverse primer 'name'
# Plasmid 2 - reverse primer 'name'
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Current revision

< Haynes Lab OWW Home | Wiki Editing

Plasmid Validation Template page

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'



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