Haynes:WikiTemp NBplasmids: Difference between revisions
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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home] | [http://openwetware.org/wiki/Haynes:TechResources#Wiki_Editing Wiki Editing] | |||
=Plasmid Validation Template page= | |||
<!--Select all of the code that is between the "TEMPLATE CODE" markers. Do not include the "TEMPLATE CODE" markers.--> | <!--Select all of the code that is between the "TEMPLATE CODE" markers. Do not include the "TEMPLATE CODE" markers.--> | ||
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'''DNA Concentration Data''' | '''DNA Concentration Data''' | ||
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{| {{table}} | {| {{table}} | ||
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| Plasmid || OD260 || OD260/280 || ng/μL | | Plasmid || OD260 || OD260/280 || ng/μL | ||
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'''Restriction Digest Table'''<br> | '''Restriction Digest Table'''<br> | ||
* | * Checked plasmid minipreps with EcoRI/PstI digests | ||
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'''DNA Sequencing Samples''' | '''DNA Sequencing Samples''' | ||
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* Submitted to DNASU on 'date' | <!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | ||
* Submitted to DNASU on: 'date' | |||
* Order Number: | |||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | <!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | ||
# Plasmid 1 - forward primer 'name' | # Plasmid 1 - forward primer 'name' | ||
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# Plasmid 2 - forward primer 'name' | # Plasmid 2 - forward primer 'name' | ||
# Plasmid 2 - reverse primer 'name' | # Plasmid 2 - reverse primer 'name' | ||
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Latest revision as of 14:57, 21 September 2013
< Haynes Lab OWW Home | Wiki Editing
Plasmid Validation Template page
DNA Concentration Data
Plasmid | OD260 | OD260/280 | ng/μL |
1. Plasmid 1 | --- | --- | --- |
2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
15 μL/lane; 1% agarose; Ladder |
DNA(plasmid) | 3.0 μL | ||
10X buffer | 1.5 | ||
EcoRI | 1.0 | ||
PstI | 1.0 | ||
dH2O | 8.5 | ||
15 μL --> 37°C/ 15 min. |
DNA Sequencing Samples
- Submitted to DNASU on: 'date'
- Order Number:
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'