Haynes:WikiTemp NBplasmids: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (13 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home] | [http://openwetware.org/wiki/Haynes:TechResources#Wiki_Editing Wiki Editing] | |||
=Plasmid Validation Template page= | |||
<!--Select all of the code that is between the "TEMPLATE CODE" markers. Do not include the "TEMPLATE CODE" markers.--> | <!--Select all of the code that is between the "TEMPLATE CODE" markers. Do not include the "TEMPLATE CODE" markers.--> | ||
<!--Copy the text--> | <!--Copy the text--> | ||
| Line 5: | Line 9: | ||
<!--TEMPLATE CODE--> | <!--TEMPLATE CODE--> | ||
'''DNA Concentration Data'''< | '''DNA Concentration Data''' | ||
<!-- Wiki code notes: The {| is the opening tag for tables. {{table}} is an OWW template that creates thin grey border lines and cell margins. | |||
<!-- Each |- starts a new row. There are three rows. bgcolor=#cfcfcf *colors* the *background* of the first row grey. --> | |||
<!-- Below that, each | starts a row of cells. --> | |||
<!-- The symbol || separates cells in a row. Replace the --- in each cell with your data. --> | |||
<!-- If you have only one plasmid, delete the |- under Plasmid 1's row, and delete the entire row for Plasmid 2.--> | |||
<!-- |} must be the last symbol (on its own line) in the table. This is the table closing tag. --> | |||
{| {{table}} | {| {{table}} | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Plasmid || OD260 || OD260/280 || ng/μL | | Plasmid || OD260 || OD260/280 || ng/μL | ||
| Line 22: | Line 28: | ||
'''Restriction Digest Table'''<br> | '''Restriction Digest Table'''<br> | ||
* | * Checked plasmid minipreps with EcoRI/PstI digests | ||
{| {{table}} border="1" cellspacing="3" | {| {{table}} border="1" cellspacing="3" | ||
| Line 30: | Line 36: | ||
<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey. --> | <!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey. --> | ||
<!-- The next two "rowspan=7" cells span all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. --> | <!-- The next two "rowspan=7" cells span all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. --> | ||
<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2 | |||
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file --> | <!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file --> | ||
<!-- is ASCII code for an invisible space. --> | <!-- is ASCII code for an invisible space. --> | ||
| Line 50: | Line 57: | ||
| || 15 μL --> 37°C/ 15 min. | | || 15 μL --> 37°C/ 15 min. | ||
|} | |} | ||
'''DNA Sequencing Samples''' | |||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | |||
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | |||
* Submitted to DNASU on: 'date' | |||
* Order Number: | |||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | |||
# Plasmid 1 - forward primer 'name' | |||
# Plasmid 1 - reverse primer 'name' | |||
# Plasmid 2 - forward primer 'name' | |||
# Plasmid 2 - reverse primer 'name' | |||
<!--TEMPLATE CODE--> | |||
Latest revision as of 14:57, 21 September 2013
< Haynes Lab OWW Home | Wiki Editing
Plasmid Validation Template page
DNA Concentration Data
| Plasmid | OD260 | OD260/280 | ng/μL |
| 1. Plasmid 1 | --- | --- | --- |
| 2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
| Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
 15 μL/lane; 1% agarose; Ladder |
| DNA(plasmid) | 3.0 μL | ||
| 10X buffer | 1.5 | ||
| EcoRI | 1.0 | ||
| PstI | 1.0 | ||
| dH2O | 8.5 | ||
| 15 μL --> 37°C/ 15 min. |
DNA Sequencing Samples
- Submitted to DNASU on: 'date'
- Order Number:
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
