Haynes:WikiTemp NBplasmids

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(New page: <!--Select all of the code that is between the "TEMPLATE CODE" markers. Do not include the "TEMPLATE CODE" markers.--> <!--Copy the text--> <!--Go back to your notebook page, click the edi...)
Current revision (17:57, 21 September 2013) (view source)
 
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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home] | [http://openwetware.org/wiki/Haynes:TechResources#Wiki_Editing Wiki Editing]
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=Plasmid Validation Template page=
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'''DNA Concentration Data'''<br>
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'''DNA Concentration Data'''
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<!-- Wiki code notes: The {| is the opening tag for tables. {{table}} is an OWW template that creates thin grey border lines and cell margins.
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{| {{table}} cellspacing="3"
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<!-- Each |- starts a new row. There are three rows. bgcolor=#cfcfcf *colors* the *background* of the first row grey.  -->
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<!-- If you have only one plasmid, delete the |- under Plasmid 1's row, and delete the entire row for Plasmid 2.-->
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{| {{table}}
|- bgcolor=#cfcfcf  
|- bgcolor=#cfcfcf  
| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
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'''Restriction Digest Table'''<br>
'''Restriction Digest Table'''<br>
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* Check plasmid minipreps with EcoRI/PstI digests
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* Checked plasmid minipreps with EcoRI/PstI digests
{| {{table}} border="1" cellspacing="3"  
{| {{table}} border="1" cellspacing="3"  
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<!-- The next two "rowspan=7" cells span all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
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| bgcolor=#cfcfcf | Reagent || Volume
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| bgcolor=#cfcfcf | Reagent  
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| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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| &nbsp; || 15 μL --> 37°C/ 15 min.
| &nbsp; || 15 μL --> 37°C/ 15 min.
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|}
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'''DNA Sequencing Samples'''
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* Submitted to DNASU on: 'date'
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* Order Number:
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<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
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# Plasmid 1 - forward primer 'name'
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# Plasmid 1 - reverse primer 'name'
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# Plasmid 2 - forward primer 'name'
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# Plasmid 2 - reverse primer 'name'
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<!--TEMPLATE CODE-->

Current revision

< Haynes Lab OWW Home | Wiki Editing

Plasmid Validation Template page

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'



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