Haynes:WikiTemp NBplasmids

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'''Restriction Digest Table'''<br>
'''Restriction Digest Table'''<br>
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* Check plasmid minipreps with EcoRI/PstI digests
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* Checked plasmid minipreps with EcoRI/PstI digests
{| {{table}} border="1" cellspacing="3"  
{| {{table}} border="1" cellspacing="3"  

Revision as of 09:56, 3 September 2013


DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on 'date'
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'
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