Haynes:WikiTemp NBplasmids

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 6: Line 6:
'''DNA Concentration Data'''
'''DNA Concentration Data'''
-
 
<!-- Wiki code notes: The {| is the opening tag for tables. {{table}} is an OWW template that creates thin grey border lines and cell margins.  
<!-- Wiki code notes: The {| is the opening tag for tables. {{table}} is an OWW template that creates thin grey border lines and cell margins.  
<!-- Each |- starts a new row. There are three rows. bgcolor=#cfcfcf *colors* the *background* of the first row grey.  -->
<!-- Each |- starts a new row. There are three rows. bgcolor=#cfcfcf *colors* the *background* of the first row grey.  -->
Line 14: Line 13:
<!-- |} must be the last symbol (on its own line) in the table. This is the table closing tag. -->
<!-- |} must be the last symbol (on its own line) in the table. This is the table closing tag. -->
-
{| {{table}} cellspacing="3"
+
{| {{table}}  
|- bgcolor=#cfcfcf  
|- bgcolor=#cfcfcf  
| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL

Revision as of 10:55, 3 September 2013


DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Check plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on 'date'
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'
Personal tools