Haynes:WikiTemp NBplasmids: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
<!--TEMPLATE CODE--> | <!--TEMPLATE CODE--> | ||
'''DNA Concentration Data'''< | '''DNA Concentration Data''' | ||
<!-- Wiki code notes: The {| is the opening tag for tables. {{table}} is an OWW template that creates thin grey border lines and cell margins. | |||
<!-- Each |- starts a new row. There are three rows. bgcolor=#cfcfcf *colors* the *background* of the first row grey. --> | |||
<!-- Below that, each | starts a row of cells. --> | |||
<!-- The symbol || separates cells in a row. Replace the --- in each cell with your data. --> | |||
<!-- |} must be the last symbol (on its own line) in the table. This is the table closing tag. --> | |||
{| {{table}} cellspacing="3" | {| {{table}} cellspacing="3" | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Plasmid || OD260 || OD260/280 || ng/μL | | Plasmid || OD260 || OD260/280 || ng/μL | ||
Revision as of 07:42, 3 September 2013
DNA Concentration Data
| Plasmid | OD260 | OD260/280 | ng/μL |
| 1. Plasmid 1 | --- | --- | --- |
| 2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Check plasmid minipreps with EcoRI/PstI digests
| Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
 15 μL/lane; 1% agarose; Ladder |
| DNA(plasmid) | 3.0 μL | ||
| 10X buffer | 1.5 | ||
| EcoRI | 1.0 | ||
| PstI | 1.0 | ||
| dH2O | 8.5 | ||
| 15 μL --> 37°C/ 15 min. |
