Haynes:WikiTemp NBplasmids

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: <!--Select all of the code that is between the "TEMPLATE CODE" markers. Do not include the "TEMPLATE CODE" markers.--> <!--Copy the text--> <!--Go back to your notebook page, click the edi...)
Line 27: Line 27:
<!-- Editing: the coding for this table is a bit more advanced. -->
<!-- Editing: the coding for this table is a bit more advanced. -->
<!-- valign="top" aligns all the text in the first row to the top.  -->
<!-- valign="top" aligns all the text in the first row to the top.  -->
 +
<!-- The | symbols on the next two lines start new cells in the same row. This does the same thing as ||, but you have to use | on new lines to set formatting for cells. -->
<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey.  -->
<!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey.  -->
-
<!-- The | symbols on the next two lines start new cells in the same row, but with no bg color. They are large cells that spans all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
+
<!-- The next two "rowspan=7" cells span all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
<!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
 +
<!-- &nbsp; is ASCII code for an invisible space. -->
|- valign="top"
|- valign="top"
-
| bgcolor=#cfcfcf | Reagent || Volume
+
| bgcolor=#cfcfcf | Reagent  
 +
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]

Revision as of 10:37, 3 September 2013


DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 --- --- ---
2. Plasmid 2 --- --- ---


Restriction Digest Table

  • Check plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.
Personal tools