Haynes:TechResources

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BioBrick Collections

• Benchling - Haynes Lab Parts & Plasmids
• iGEM Registry - Haynes Lab Parts
• Dr. Haynes' parts and vectors
• Dr. Haynes' primers
• René's primers, oligos, and parts
• Brady's Parts

Chemicals, Etc.

• Chemical Inventory Spreadsheet: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).
  
• Compressed Gas: Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
• Dry Ice: Located in PSG 106 at the DCB Stockroom

Literature

• PubMed via ASU Libraries - navigating to PubMed through the ASU Libraries website will give you ASU password-protected access to scientific journals, free of charge

Ordering

• Ordering Spreadsheet - CURRENT: Requests for consumables, DNA synthesis, and small instruments are listed here
• Ordering Spreadsheet - 2011-2013: older orders
• SunRISE Ordering System: Authorized lab members can log-in to SunRISE to place orders
• DNA Sequencing - SOLS DNA Lab [show][hide]

• • Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube
  • Ordering Information
  • Order Forms

• DNA Sequencing - DNASU @Biodesign [show][hide]

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Safety

• PDF of the current, long version of the Haynes Lab SOP
• Environmental Health & Safety at ASU
• Biohazard & Chemical Waste Pick-up Request: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals)
• Individual Training Record: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.

Shared Equipment

Sign-up Calendar
for all shared instruments

Burrrt Reynolds: -150°C Cryogenic Freezer
Model: Sanyo MDFC2156VANC
Location: ISTB4, room 231, linear equipment room
Status: Burrrt is up and running!

Lucille: Light Cycler 480
Model: Roche 5015278001
Location: ISTB4, room 231, south shared workspace
Status: Online

• User technical information
• Software downloads
• Design a UPL assay - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost (comparable to SYBR Green).

Plato: BioTek Plate Reader
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy
Location: ISTB4, room 231, south shared workspace
Status: Online

Wiki Editing

• Basic Wiki editing tips - from the ASU BME100 course
• Plasmid Validation Template Page - OWW notebook copy-paste code for DNA conc., restriction digests, etc.
• Excel to Wiki table converter: here's a neat tool that let's you paste data from Excel into a form and covert it into Wiki code by clicking a button. http://excel2wiki.net/wikipedia.php